Composite

Part:BBa_K4187024

Designed by: Martin Pezous   Group: iGEM22_Ionis_Paris   (2022-10-07)


Light inducible depolymerization of Polylactate (PLA) into lactate

This part is composed of two composite parts: the Light-inducible DNA-binding protein (EL222) composite part BBa_K4187020 and the Polylactate depolymerase (PLAase) under a blue light inducible promoter (Pblind) composite part BBa_K418721. This part aims to degrade polylactate on demand.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 563
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 133
    Illegal NgoMIV site found at 1027
    Illegal NgoMIV site found at 1065
    Illegal NgoMIV site found at 1074
    Illegal NgoMIV site found at 1300
    Illegal AgeI site found at 1099
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1560


[edit]
Categories
Parameters
None